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KMID : 1161519980020020269
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Animal Cells and Systems
1998 Volume.2 No. 2 p.269 ~ p.275
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Establishment of transgenic drosophila bearing metallothionein-lacZ fusion gene and its practical use
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Kim Young-Shin
Park Hyun-Sook
Yoo Mi-Ae
Lee Won-Ho
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Abstract
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The reporter plasmid pMT?lacZ containing the metallothionein (MT) promoter region (?320∼+58 with respect to the transcription initiation site) fused to the lacZ gene in a P?element vector was constructed. Transgenic Drosophila bearing the MT?lacZ fusion gene were established by P?element mediated transformation. Expression of the MT?lacZ fusion gene in transformants was examined during development. By treatment with low concentration of cadmium (≥10μM) or paraquat (≥50μM), increased expression of β?galactosidase was shown in fat body, brain lobe, and ganglion transgenic larval tissues. The results show that transformants bearing the MT?lacZ fusion gene are useful for further studies on the mechanism of regulation of MT gene expression and for monitoring toxic metals
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KEYWORD
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Metallothionein, Transgenic Drosophilamelanogaster, Cadmium, Paraquat
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